1. Occupational Therapists’ Perspectives on Community Implementation of Interventions Unfamiliar to Older Adults
Mao, H.-F., Tsai, A. Y.-J., Yeh, H.-C., & Chang, L.-H. (2024).The Gerontologist, 65(2).Read this article
As evidence-based interventions (EBIs) for older adults expand, successful community delivery depends on both protocol adherence and responsiveness to context. This study explored the real-world implementation of a complex cognitive EBI—Multiple-component Cognitive Intervention using Simulated Everyday Tasks (MCI-SET)—from the perspective of occupational therapists in Taiwan. A convergent mixed-methods design was used. Quantitative data from 47 therapists were collected using the Perceived Fidelity Questionnaire (PFQ), while qualitative data were gathered through two World Café discussions. Results showed therapists rated community-oriented skills as more important and demonstrated greater proficiency and adherence in these areas. Qualitative analysis emphasized the need for rapport-building, strategic program introduction, and tailoring interventions to local needs. Integrated findings highlight the importance of community engagement skills and the adaptability of interventions to support fidelity and sustainability. These findings underscore the need for dynamic fidelity frameworks and tailored training that includes adaptation strategies responsive to community contexts.
隨著政府擴大高齡者相關的實證介入（evidence-based interventions）推廣，成功在社區中執行相關介入需同時注重治療師是否能夠遵循方案的原則及同時回應社區的需求。本研究探討了一項複合式認知介入「模擬日常任務的多元複合認知介入（Multiple-component Cognitive Intervention using Simulated Everyday Task）」在台灣的實施情況，聚焦於職能治療師對於方案保真度、執行挑戰與策略的看法。我們採用收斂式混合方法設計，透過主觀保真度問卷（perceived fidelity questionnaire）調查47位職能治療師，並透過兩場「世界咖啡館」座談收集質性資料。結果顯示，治療師認為與社區互動相關的能力比認知方案介入技巧更重要，且在社區相關之技巧的熟練度與執行率也較高。質性資料進一步指出，治療師需透過策略性介紹、調整活動以貼近在地需求、並與社區建立關係來提升長輩的參與與執行效果。結果強調，治療師的社區參與能力與介入彈性是實施與永續推動的重要關鍵，並呼籲未來在治療師訓練中納入動態忠實性的概念與調整策略，以因應不同社區脈絡需求。
2. Cross-cultural adaptation and psychometric validation of Importance of Good Death-Indonesian version for patients with advanced cancer
Sulistyarini, W. D., Fan, S. Y., Huang, M. C., Effendy, C., Xu, D., Chen, T. J., & Kao, C. Y. (2025).Asia-Pacific journal of oncology nursing, 12, 100798. Read this article
Abstract:
Objective: To adapt and test the psychometric properties of the Importance of Good Death Questionnaire for Indonesian patients with advanced cancer.
Methods: The original instrument underwent a rigorous process of forward and backward translation, complemented by an expert panel review to ensure cultural and conceptual equivalence and to evaluate content validity. A total of 447 patients with advanced cancer were recruited via convenience sampling from two government hospitals in Solo and Samarinda, Indonesia, between September 2022 and February 2023. The sample was then divided into two groups. Sample 1 (n = 265) was used for exploratory factor analysis to establish the initial factor structure of the Indonesian version, and Sample 2 (n = 182) was used for confirmatory factor analysis to validate construct validity. No significant differences in demographics or clinical variables were observed between the two samples.
Results: Following the expert panel review, the instrument underwent exploratory factor analysis and confirmatory factor analysis, resulting in a final Indonesian version containing 24 items across five factors: comfort, relationship closure, preparation for death, support from others, and life meaning. The five-factor model accounted for 59.1% of the total variance. Specifically, comfort explained 40% of the variance, relationship closure explained 9.2%, preparation for death explained 5%, support from others explained 2.8%, and life meaning explained 2.1%. Internal consistency was excellent, with an overall Cronbach’s alpha of 0.91 and factor-specific alpha values ranging from 0.73 to 0.93.
Conclusions: The study results indicate that the Importance of Good Death-Indonesian version has good psychometric properties and is a valid and reliable instrument to assess the importance of good death in an Indonesian advanced cancer population. In nursing practice, the instrument can guide culturally responsive end-of-life care by identifying patients’ priorities across five domains. This enables nurses to develop individualized care plans, address pain and symptom needs, facilitate meaningful family interactions, support spiritual or practical preparation for death, and inform palliative care decision-making.
3. USPPAR is a cost-effective, scalable, and highly sensitive single-cell RNA sequencing workflow compatible with diverse specimens
Hsueh, Y. W., & Chiang, P. M. (2025).PLoS biology, 23(12), e3003537. Read this article
Current scRNA-seq methods often trade cell throughput for sensitivity and lack universal tissue compatibility. We introduce USPPAR, a unified framework that overcomes these barriers through optimized split-pool barcoding and a breakthrough terminal deoxynucleotidyl transferase (TdT) reaction.By utilizing a precise low-Co2+ environment devoid of other metal-ion components, USPPAR efficiently polydeoxyadenylates intractable DNA ends, achieving sensitivity comparable to low-throughput methods at high cell capacity. Furthermore, we developed a dual-action RNA protection strategy: partially chelated copper ions serve as a broad-spectrum nuclease inhibitor, while lysine conjugation permanently inactivates RNases during nuclear extraction. This allows high-quality recovery from "difficult" samples like the pancreas and spleen. Requiring no specialized equipment and remaining fully open-source, USPPAR provides a cost-effective, high-resolution solution for profiling diverse animal and plant tissues.
目前的單細胞測序常面臨高通量與靈敏度難以兼得，以及組織樣本不通用的困境。我們開發出全新的 USPPAR 系統，透過突破性的 DNA 末端加尾技術與核酸酶抑制機制，成功打破瓶頸。該系統同時具備高通量與高靈敏度優勢，且無需昂貴設備並完全開源，讓所有人能不受環境限制，統一分析各類動植物組織，提供高效、低成本的解決方案。
