獲得圖書館OA補助文章之介紹 (服務辦法)
  • 1. Z-Complementary Code Sets With Flexible Lengths From Generalized Boolean Functions.

    Wu, S. W., Şahin, A., Huang, Z. M., & Chen, C. Y. (2021). IEEE Access, 9, 4642-4652. Cite this article

    In the literature, most Z-complementary code set (ZCCS) constructions based on generalized Boolean functions lead to sequences of power-of-two length. In this study, we show that our proposed direct constructions result in ZCCSs of both power-of-two length and non-power-of-two length. Since the monomials of degrees more than 2 are employed in the proposed constructions, more ZCCSs can be obtained. The constructed ZCCSs admit the theoretical upper bound on the size for a ZCCS when the sequence length is a power of two. Also, the corresponding peak-to-average-power ratio (PAPR) is theoretically upper-bounded when a sequence in the set is used in OFDM. The proposed constructions extend the applications of ZCCSs in practical communication systems, e.g., multicarrier CDMA (MC-CDMA) system, by offering flexible sequence lengths, various set sizes, and bounded PAPR.


  • 2. Anti-IL-20 Antibody Protects against Ischemia/Reperfusion-Impaired Myocardial Function through Modulation of Oxidative Injuries, Inflammation and Cardiac Remodeling.

    Tsai, K.-L., Chou, W.-C., Cheng, H.-C., Huang, Y.-T., Chang, M.-S., & Chan, S.-H. (2021). Antioxidants, 10(2), 275. Cite this article

    We confirmed that IL-20 antibody injection at the moment of reperfusion reduces I/R-caused oxidative injuries, apoptosis, inflammation, as well as the elevation in markers of cardiac fibrosis and remodeling. We also proved that IL-20 antibody injection attenuates cardiac functional impairment, including the repressed EF and FS, the increased left ventricular end-diastolic and end-systolic volumes, and plasma markers of cardiac injury and heart failure. This study elucidates that IL-20 antibody might be favorable in lessening reperfusion injury in the treatment of AMI.


  • 3. The Spatial-Temporal Distribution of GOCI-Derived Suspended Sediment in Taiwan Coastal Water Induced by Typhoon Soudelor.

    Chau, P. M., Wang, C.-K., & Huang, A.-T. (2021). Remote Sensing, 13(2), 194. Cite this article

    We use high temporal resolution Geostationary Ocean Color Imager (GOCI) to monitor the spatial–temporal distribution of suspended sediment (SS) along the coastal waters of northern Taiwan which was affected by Typhoon Soudelor from 8 to 10 August 2015. The results show that SS concentration increased during 11–14 August and then decreased during 19–22 August. The mouths of the Dajia River and Tamsui River were hotspots of SS, ranging from 9 to 15 g/m3, during the two post-typhoon periods. It is also shown that maximum SS occurred in the west and east coastal water during 11–14 August and 19–22 August, respectively. Furthermore, an exponential decay model was used to compute the time when 90% of typhoon-induced SS was dissipated after Typhoon Soudelor. It was found that the mouths of the Tamsui River and Heping River had the longest time, with a range of 360–480 h.

    本文以高時間解析度GOCI衛星影像,觀測北台灣沿岸海水懸浮泥沙濃度(SS)受2015年8月蘇迪樂颱風影響之時空分佈。在颱風過後,SS分別於8月11–14日觀測期間上升,8月19–22日觀測期間下降,且大甲溪與淡水河之河口為高SS之熱區,達9–15 g/m3。以指數衰減模型計算減少90%颱風誘發的SS所需時間,發現淡水河與和平溪河口需要360–480小時。

  • 4. An aptamer interacting with heat shock protein 70 shows therapeutic effects and prognostic ability in serous ovarian cancer.

    Lin, C.-N., Tsai, Y.-C., Hsu, C.-C., Liang, Y.-L., Wu, Y.-Y., Kang, C.-Y., Lin, C.-H., Hsu, P.-H., Lee, G.-B., & Hsu, K.-F. (2021). Molecular Therapy - Nucleic Acids, 23, 757-768. Cite this article

    In this study, we aim to clarify the novel aptamer, Tx-01, clinical role and possible molecular mechanisms of Tx-01 in Ovarian cancer. Immunostaining and statistical analysis were performed to detect the interaction of Tx-01 and heat shock protein 70/Notch1 intracellular domain in OvCa. The in vitro and in vivo experiments demonstrated the potential mechanisms of Tx-01. Tx-01 reduced serous OvCa OVCAR3 cell migration and invasion and inhibited HSP70 nuclear translocation by interrupting the intracellular HSP70/NICD interaction. Furthermore, Tx-01 suppressed serous-type OVCAR3 cell tumor growth in vivo. Tx-01 acts as a prognostic factor through its interaction with membrane-bound HSP70 on ascetic circulating tumor cells and is reported to be involved in natural killer (NK) cell recognition and activation. Our data demonstrated that Tx-01 interacted with HSP70 and showed therapeutic and prognostic effects in serous OvCa. Tx-01 might be a potential inhibitor for use in serous OvCa treatment.

    在這個研究中,我們發現新穎的適體TX-01跟HSP70在卵巢癌中有做結合。TX-01可以抑制卵巢癌OVCAR3細胞的移動及入侵, 同時抑制HSP70細胞核的移動 , 在小鼠上抑制OVCAR3的腫瘤生長。透過卵巢癌病人的腹水當中HSP70的結合, 可以作為治療預後的指標。因此我們認為TX-01的適體透過HSP70的結合可以在漿液性卵巢癌當中,具有作為治療以及預後預測的角色。

  • 5. Diversified Phenomena in Metal- and Transition-Metal-Adsorbed Graphene Nanoribbons.

    Lin, S.-Y., Tran, N. T. T., & Lin, M.-F. (2021). Nanomaterials, 11(3), 630. Cite this article

    In this study, the significant similarities and differences among Bi-/Al-/Ti-/Fe-/Co-/Ni-adsorbed graphene nanoribbons (GNRs) are thoroughly investigated by using the first-principles calculations. The main characterizations include the adsorption sites, bond lengths, stability, band structures, charge densities, spin- and orbital-projected density of states, and magnetic configurations. Furthermore, there exists a transformation from finite gap semiconducting to metallic behaviors, accompanied by the nonmagnetism, antiferromagnetism, or ferromagnetism. The proposed theoretical framework could be generalized to explore other emergent 1D and 2D materials.

    使用第一原理計算,研究其Bi // Al- / Ti- / Fe- / Co- / Ni吸附的石墨烯奈米帶(GNR)之間的顯著差異。主要特徵為吸附位、鍵長、穩定性、能帶結構、電荷密度、自旋及軌道投影態密度與磁性構型。此外,伴隨著非磁性,反鐵磁性或鐵磁性,存在有限間隙半導體到金屬行為之轉變。此結果概括了理論性框架,及探索研究其它新興之1D與2D材料。

  • 6. Nanomedicine-Based Strategies Assisting Photodynamic Therapy for Hypoxic Tumors: State-of-the-Art Approaches and Emerging Trends.

    Shih, C.-Y., Wang, P.-T., Su, W.-C., Teng, H., & Huang, W.-L. (2021).Biomedicines, 9(2), 137. Cite this article

    Since the first clinical cancer treatment in 1978, photodynamic therapy (PDT) technologies have been largely improved and approved for clinical usage in various cancers. Due to the oxygen-dependent nature, the application of PDT is still limited by hypoxia in tumor tissues. Thus, the development of effective strategies for manipulating hypoxia and improving the effectiveness of PDT is one of the most important area in PDT field. Recently, emerging nanotechnology has benefitted progress in many areas, including PDT. In this review, we will discuss the state of the art of nanomedicine-based approaches for assisting PDT for treating hypoxic tumors, mainly based on oxygen replenishing and oxygen dependency diminishing strategies. We will emphasize emerging trends about the use of nanoscale metal–organic framework materials and the combination of PDT with immunotherapy. We further discuss future perspectives and challenges associated with these trends in both the aspects of mechanism and clinical translation.


  • 7. Phage Digestion of a Bacterial Capsule Imparts Resistance to Two Antibiotic Agents.

    Luo, C.-H., Hsu, Y.-H., Wu, W.-J., Chang, K.-C., & Yeh, C.-S. (2021).Microorganisms, 9(4), 794. Cite this article

    Bacteriophages are viruses that specifically infect bacteria, which have developed extraordinary proteins for recognizing and degrading their host. Inspired by the remarkable development of viral proteins, phage-derived proteins have been used for clinical treatment of multi-drug resistant (MDR) bacteria infection. For example, the polysaccharide depolymerase or endolysins were used to attenuate bacterial cell walls or to destroy the bacterial membrane. More recent efforts have addressed bacterial selection to treat drug-stress or phage infection. We, then, propose a combination strategy of co-treating MDR Acinetobacter baumannii with phage endolysin derived peptide and polysaccharide depolymerase (the tail fiber molecules). In the study, we surprisingly observed a temporary increase in resistance to colistin, used as the last-resort antibiotic for MDR Gram negative bacterial infection. Evaluation of the phage attacking behavior suggests the temporary increased resistance may be associated with an increased risk of drug-resistance during phage-antibiotic synergy in clinical MDR A. baumannii treatment.


  • 8. Computer-Assisted System in Stress Radiography for Anterior Cruciate Ligament Injury with Correspondent Evaluation of Relevant Diagnostic Factors.

    Wang, C.-K., Lin, L.-C., Sun, Y.-N., Lai, C.-S., Chen, C.-H., & Kao, C.-Y. (2021).Diagnostics, 11(3), 419. Cite this article

    Retrospective research for 177 patients (129 with completely torn ACLs confirmed on magnetic resonance imaging) at our institution was performed. The anterior tibial translations with four different methods were measured by a computer-assisted system. Mean anterior tibial translations were significantly increased in the patients with a completely torn ACL, with diagnostic accuracies ranging from 66.7% to 75.1%. The diagnostic accuracy of method four was negatively associated with the time interval between stress radiography and MRI as well as force-joint distance on stress view, and not significantly associated with age, gender, flexion angle, intercondylar distance, and force-joint angle.


  • 9. Anti-Inflammatory Principles from the Needles of Pinus taiwanensis Hayata and In Silico Studies of Their Potential Anti-Aging Effects.

    Kuo, P.-C., Li, Y.-C., Kusuma, A. M., Tzen, J. T. C., Hwang, T.-L., Ye, G.-H., Yang, M.-L., & Wang, S.-Y. (2021).Antioxidants, 10(4), 598. Cite this article

    Pinus needle tea, which is very popular in Eastern countries, is claimed to have significant anti-aging effects. In this study, five undescribed compounds as well as seventy-two known compounds were characterized from the methanol extracts of P. taiwanensis needles. Most of the isolates were examined for their anti-inflammatory bioactivity, and six compounds exhibited significant inhibitions on superoxide anion generation and elastase release. These ingredients were subjected to docking computing to evaluate their binding affinity on the ghrelin receptor to explore the potentials of anti-aging effect. Three tested compounds formed a stable complex with the ghrelin receptor via hydrogen bonds and different types of interactions, and it suggested these flavonoids to be responsible for the potential anti-aging effects of pine needle tea.


  • 10. Electrosynthesized Ni-P nanospheres with high activity and selectivity towards photoelectrochemical plastics reforming.

    Lin, C.-Y., Huang, S.-C., Lin, Y.-G., Hsu, L.-C., & Yi, C.-T. (2021). Applied Catalysis B: Environmental, 296, 120351. Cite this article

    Photoelectrochemical reforming of plastic waste offers an environmentally-benign and sustainable route for hydrogen generation. Nonetheless, little attention was paid to develop electrocatalysts that can efficiently and selectively catalyze oxidative transformation of valueless plastic wastes into valued chemicals. Herein, we report on facile electrosynthesis of nickel-phosphorus nanospheres (nanoNi-P), and their versatility in catalyzing hydrogen generation, water oxidation, and reforming of polyethylene terephthalate (PET). Notably, composite of nanoNi-P with carbon nanotubes (CNT/nanoNi-P) requires −180 mV overpotential to drive hydrogen generation at -100 mA cm−2. Besides, CV-activated nanoNi-P (nanoNi-P(CV)) was shown to be capable of reforming PET into formate with high selectivity (Faradic efficiency= ∼100 %). Efficient and selective generation of hydrogen and formate from PET reforming is realized utilizing an Earth-abundant photoelectrochemical platform based on nanoNi-P(CV)-modified TiO2 nanorods photoanode and CNT/nanoNi-P cathode. This work paves a path for developing artificial leaf for simultaneous environmental mitigation and photosynthesis of renewable fuels and valued chemicals.


  • 11. Association of Primary Treatment Modality for Advanced-Stage Oropharyngeal Squamous Cell Carcinoma With Survival Outcomes.

    Tsai, M.-H., Cheng, Y.-J., Pao, T.-H., Hsueh, W.-T., Chen, H. H. W., & Wu, Y.-H. (2021). JAMA Network Open, 4(6), e2112067-e2112067. Cite this article

    This retrospective comparative effectiveness analysis used data from the population-based Taiwan Cancer Registry. Included patients were diagnosed with clinical stage III or IV oropharyngeal squamous cell carcinoma from 2007 to 2015 and were identified from the registry. Among 1180 patients, 694 patients (58.8%) were in the definitive chemoradiotherapy group and 486 patients (41.2%) were in the upfront surgical treatment group. The mean age was 54.59 years. Primary treatment with an upfront surgical procedure was associated with a decreased risk of death during the study period (HR: 0.81; P = .02). However, when adjusted for age, subsite, histological grade, and T and N classification, upfront surgical treatment was no longer associated with an increased risk of death during the study period (HR: 0.96; P = .70). Progressionfree survival was worse in the group receiving upfront surgical treatment than in the group receiving chemoradiotherapy (HR, 1.64; P = .02), and this difference persisted after adjusting for other factors associated with prognosis(HR, 1.72; p = .01). This study found that definitive chemoradiotherapy was associated with effectiveness that was comparable with that of upfront surgical treatment when adjusted for baseline factors associated with prognosis. These findings suggest that definitive chemoradiotherapy should be considered to avoid accumulating toxic effects associated with surgical treatment and chemoradiotherapy.

    本研究為回溯性之比較效益研究。我們採用台灣癌症登記資料庫的數據, 2007年至2015年第III或IV期口咽鱗狀細胞癌, 1180例患者中,有694例(58.8%)接受了根治性放射治療併化學治療,486例(41.2%)接受手術治療。中位(四分間距)追蹤時間為3.62(1.63-5.47)年,大多數患者為男性(1052 [89.1%])、原發於扁桃線(712例[60.3%]),組織呈中度分化(604例[51.2%]),臨床期別為N2(858例[72.7%])與IVA疾病(938患者[79.5%])。於研究期間中,接受手術治療與較低死亡風險相關(HR 0.81;p = .02)。但經年齡、原發部位、分化程度、及T和N分期調整後,則不再與死亡風險相關(HR,0.96;P = .70)。接受手術治療的無進展生存期較差(HR,1.64;P = .02),且調整了與已知預後因子(即年齡、原發部位、分化程度、及T和N分期)後,仍有顯著差異(HR,1.72;P = 0.01)。結論與相關性:我們的分析顯示,在校正已知預後因子後,根治性放射治療併化學治療與手術治療在局部晚期口咽癌的效果相當。我們認為應優先考慮根治性放射治療併化學治療,以避免手術與根治性放射治療併化學治療的三重毒性。

  • 12. Development of spatially varying groundwater-drawdown functions for land subsidence estimation.

    Chu, H.-J., Ali, M. Z., Tatas, & Burbey, T. J. (2021). Journal of Hydrology: Regional Studies, 35, 100808. Cite this article

    Land subsidence caused by groundwater overexploitation is a critical global problem. The spatial distribution of land subsidence is crucial for effective environmental management and land planning in subsidence prone areas. Because of the nonlinear relationship between subsidence and drawdown due to groundwater exploitation in heterogeneous aquifers, a spatial regression (SR) model is developed to effectively estimate nonlinear and spatially varying land subsidence. Considering various data inputs in the Choshui River alluvial fan, the SR model offers a robust method for accurately estimating the spatial patterns of subsidence using only drawdown as input data.


  • 13. Neurodevelopment regulators miR-137 and miR-34 family as biomarkers for early and adult onset schizophrenia.

    Chen, B.-Y., Lin, J.-J., Lu, M.-K., Tan, H.-P., Jang, F.-L., & Lin, S.-H. (2021). npj Schizophrenia, 7(1), 35. Cite this article

    Early-onset schizophrenia (EOS) may have stronger familial aggregation and a more severe outcome than adult-onset schizophrenia (AOS). MicroRNA (miRNA) takes on dual roles as a genetic and epigenetic modulator, which mediate the influence of genetic risk. Neurological soft signs (NSS) are neurological abnormalities that may be intermediate phenotypes or endophenotypes for schizophrenia. We aimed to identify a set of aberrant neurodevelopmental-related miRNAs that could serve as potential biomarkers for EOS or schizophrenia with NSS. Receiver operating characteristic (ROC) analysis showed miR-137 dysregulation was correlated with almost all NSS subscales and, when EOS patients with NSS, miR-137 expression discriminated these patients from healthy controls to a greater extent (AUC = 0.957). MiR-34b had the highest ability to discriminate between EOS and AOS (AUC = 0.810), which may indicate different etiological pathways to disease onset. These findings support the potential for neurodevelopmental-related miRNAs to be used as indicators of vulnerability to EOS.
    Keywords: schizophrenia, epigenetic, microRNA, neurological soft signs, endophenotype, neurodevelopment.

    早發性思覺失調症患者相較於成人才發病的患者可能有較高的家族聚集性與較嚴重的症狀。而微型核醣核酸具備遺傳與表觀遺傳的雙重角色,軟性神經學症狀可能為思覺失調症的內在表現型。microRNA-137可鑑別早發性思覺失調症伴隨軟性神經學症狀患者與健康者(AUC = 0.957)。而microRNA-34b則能於早發性與成人型思覺失調症患者之間有良好的鑑別能力(AUC = 0.810)。本研究成功利用一組與神經發展相關的微型核糖核酸(microRNA-137與microRNA-34家族)作為鑑別早發型思覺失調症患者的潛在生物標記。

  • 14. Etoposide Triggers Cellular Senescence by Inducing Multiple Centrosomes and Primary Cilia in Adrenocortical Tumor Cells.

    Teng, Y.-N., Chang, H.-C., Chao, Y.-Y., Cheng, H.-L., Lien, W.-C., & Wang, C.-Y. (2021). Cells, 10(6), 1466. Cite this article

    Etoposide (ETO) has been used in treating adrenocortical tumor (ACT) cells. In this study, we showed that ETO treatment at 10 μM inhibited ACT cell growth by inducing cellular senescence rather than apoptosis. Several markers of cellular senescence, including activated senescence-associated β-galactosidase activity, elevated levels of p53, and down-regulation of lamin B1, were observed. We further found that ETO induced multiple centrosomes. The inhibition of multiple centrosomes accomplished by treating cells with either roscovitine or centrinone. DNA-PK-Chk2 signaling was activated by ETO treatment; inhibition of this signaling cascade alleviated multiple ETO-induced centrosomes and primary cilia followed by reducing cellular senescence. Autophagy was also triggered by ETO treatment. The inactivation of DNA-PK-Chk2 signaling reduced ETO-triggered autophagy; however, the inhibition of autophagy did not affect DNA-PK-Chk2 activation. Thus, ETO activated the DNA-PK-Chk2 cascade to facilitate autophagy. The activated autophagy further induced multiple centrosomes and primary cilia followed by triggering senescence.

    本研究發現當以濃度10 µM的etoposide (ETO)處理細胞時,會誘發cellular senescence,伴隨SA-β-GAL酵素活性會增加、p53上升和lamin B1下降。ETO會導致multiple centrosomes。ETO也會活化DNA-PK-Chk2 signaling,抑制此signaling會減少multiple centrosomes及primary cilia進而減少senescence,autophagy也受到相同的訊息路徑的調控,當抑制DNA-PK-Chk2 signaling會減少ETO所誘發的autophagy,而當抑制autophagy不會影響DNA-PK-Chk2 signaling的活化。因此ETO活化DNA-PK-Chk2 signaling以促進autophagy,進而誘導multiple centrosomes及primary cilia觸發senescence。

  • 15. Trajectory of Food Insecurity and Its Association with Longitudinal Mental Health and Sleep Outcomes in Adolescents from Economically Disadvantaged Families.

    Lee, T.-H., Kuo, J.-H., Liu, C.-Y., Yu, Y.-F., Strong, C., Lin, C.-Y., Lee, C.-T., & Tsai, M.-C. (2021). Nutrients, 13(5), 1696. Cite this article

    There is a paucity of research examining how the change of food insecurity (FI) over time is related to mental health and sleep outcomes in Taiwanese adolescents. The data come from the Taiwan Database of Children and Youth in Poverty, which is a national longitudinal project measuring FI in five survey waves (2009-2017). We employed group-based trajectory modeling to classify 1921 participants aged 12-18 years into four FI trajectory groups: persistently low FI (24.8%), persistently moderate FI (64.7%), declining from high to low FI (4.1%), and food-secure groups (6.4%). Furthermore, a generalized estimating equation analysis was conducted the persistently moderate FI group was more likely to have mental (β = 0.30, [95% confidence interval 0.21-0.38]) and sleep problems (β = 0.39, [0.29-0.48]), as compared to food-secure adolescents. Our study describes the FI profile of adolescents from economically disadvantaged families. Healthcare providers can aid adolescents and provide guidance when appropriate.


    很少有研究調查糧食匱虞(FI)隨時間的變化如何與台灣青少年的心理健康和睡眠結果相關。數據來自台灣貧窮兒童與青少年數據庫,這是一個全國性縱貫計畫,在五個調查波(2009-2017)中測量FI。我們採用群組化軌跡模型將 1921名 12至18 歲的參與者分為四個 FI 軌跡組別,分別是持續低度FI (24.8%)、持續中度FI (64.7%)、從高到低FI (4.1%) 和糧食安全組(6.4%)。接續進行廣義估計方程分析,發現對比糧食安全的青少年,持續中等 FI 組更可能有心理(β = 0.30,[95% 信賴區間 0.21-0.38])和睡眠問題(β = 0.39,[0.29-0.48])。我們的研究描述了來自經濟弱勢家庭的青少年的 FI 概況。醫療照顧提供者可以幫助青少年並在適當時提供指導。

  • 16. Sitting Posture during Prolonged Computer Typing with and without a Wearable Biofeedback Sensor.

    Kuo, Y.-L., Huang, K.-Y., Kao, C.-Y., & Tsai, Y.-J. (2021). International Journal of Environmental Research and Public Health, 18(10), 5430. Cite this article

    Prolonged sitting combined with an awkward posture might contribute to the increased risks of developing spinal pain. Maintaining an upright sitting posture is thus often suggested. This study aimed to investigate the effects of a wearable biofeedback sensor on maintaining an upright sitting posture. Twenty-one healthy young adults were recruited and performed a 1-h computer typing task twice, with and without using the active biofeedback device. The sagittal spinal posture during computer typing was measured using a three-dimensional motion analysis system. Using the wearable bio-feedback sensor significantly decreased the neck flexion, thoracic kyphotic, and pelvic plane angles compared with not using the sensor. Computer users and sedentary workers may benefit from using wearable biofeedback sensors to actively maintain an upright sitting posture during prolonged deskwork.


  • 17. Applying an Adaptive Signal Identification Method to Improve Vessel Echo Detection and Tracking for SeaSonde HF Radar.

    Chuang, L. Z.-H., Chen, Y.-R., & Chung, Y.-J. (2021). Remote Sensing, 13(13), 2453. Cite this article

    To enhance remote sensing for maritime safety and security. High-frequency (HF) radar systems are a type of mainstream technology widely used in international marine remote sensing. However, effectively recognizing targets in spectra with intrinsic strong disturbance echoes and random environmental noise is still challenging. To avoid the above problem, this paper proposes an adaptive signal identification method to detect target signals based on a rapid and flexible threshold. By integrating a watershed segmentation algorithm, the subsequent direction result can be used to automatically compute the direction of arrival of the targets. To assist in the orientation of the object, forward intersections are integrated with the technique. Hence, the proposed technique can effectively recognize vessel echoes with automatic identification system (AIS) verification. Experiments have demonstrated the promising feasibility of the proposed method’s performance.
    Keywords: high-frequency (HF) radar; adaptive signal identification (ASI); vessel detection; direction finding; automatic identification system (AIS)

    關鍵字:高頻雷達; 調適性偵測技術; 船舶偵測; 方位辨識; 自動辨識系統

  • 18. Sex differences in the diagnosis of autism spectrum disorder and effects of comorbid mental retardation and attention-deficit hyperactivity disorder.

    Tang, C. H., Chi, M. H., Hsieh, Y. T., Lee, T. I., Tai, Y. C., Lien, Y.-J., Yang, Y. K., & Chen, P. S. (2021). Journal of the Formosan Medical Association. Cite this article

    The association between sex and diagnostic behavior of autism spectrum disorder (ASD), and the effects of comorbid mental retardation (MR) and attention-deficit hyperactivity disorder (ADHD), were explored. The incidence was higher in males than in females across ages. Comorbid MR or ADHD increased the incidence of ASD in both sexes; comorbid MR or ADHD also decreased the male to female hazard ratio of ASD, with no significant differences in the incidence density of ASD between sexes. ADHD delayed diagnosis in both sexes. The results indicating unique vulnerabilities to MR/ADHD or under-identification of high-functioning females with ASD subjects. Increasing the diagnostic sensitivity of ASD in those with comorbid ADHD is important due to a delayed diagnostic age in this group.


  • 19. Taxonomic and Functional Dysregulation in Salivary Microbiomes During Oral Carcinogenesis.

    Chen, J.-W., Wu, J.-H., Chiang, W.-F., Chen, Y.-L., Wu, W.-S., & Wu, L.-W. (2021). Frontiers in Cellular and Infection Microbiology, 11(861). Cited this article

    In this report, we systematically characterized the compositions and ecological drivers of salivary microbiome in the cohorts of orally healthy, non-recurrent oral verrucous hyperplasia (OVH), and OVH–associated oral cancer at taxonomic and function levels, and compared them with the re-analysis of publicly available datasets through using bacterial 16S rRNA amplicon sequencing and in silico function analysis by PICRUSt2 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States 2). Diversity analyses showed the decline of core species numbers and the deregulation of metabolic pathways as host oral health deteriorated. Partitioned beta-diversity revealed an extremely high species turnover but low function turnover. Functional beta-diversity in saliva microbiome shifted from turnover to nestedness during oral carcinogenesis, which was not observed at taxonomic levels. Moreover, metabolic pathways of nucleotides, amino acids, fatty acids, and cell structure are strongly related with the disease progression. Using predicted functional profiles to elucidate the correlations to the oral health status thus shows superior performance than those using taxonomic data. Together, this study not only advances our understanding of the oral ecosystem in relation to oral carcinogenesis but also provide a new direction for using microbiome-based tools to study the interplay of the oral microbiome, metabolites, and host health.


  • 20. New Structural and Single Nucleotide Mutations in Type I and Type II Collagens in Taiwanese Children With Type I and Type II Collagenopathies.

    Tsai, M.-C., Chou, Y.-Y., Li, C.-Y., Wang, Y.-C., Yu, H.-W., Chen, C.-H., & Chen, P.-C. (2021). Frontiers in Genetics, 12(1268) Cited this article

    Collagenopathy is a rare genetic condition characterized by abnormality in either collagen structure or metabolism. Through whole exome sequencing (WES) complemented with multiplex ligation-dependent probe amplification, we identified the genetic etiologies for six cases with osteogenesis imperfecta (OI) in COL1A1 (p.T1298N, p.Q1280Pfs∗51, and p.G557Vfs∗23) and COL1A2 (c.1-1677_133-441del) as well as three cases with spondyloepiphyseal dysplasia congenita in COL2A1 (p.G1041S, p.G654S, and p.G441A). Co-occurrence of COL1A1 and WNT1 mutations was found in a patient with a mild OI phenotype but severe osteoporosis. These findings extended the pathogenic variant spectrum of COL1A1, COL1A2, and COL2A1 for type I and type II collagenopathies. Although WES provides a fast and accurate method to identify the genetic causes, its limitation of detecting CNVs because of variable capturing uniformity should be kept in mind when interpreting the results. We demonstrate that multiple genetic characterizing technologies can provide an accurate and efficient molecular diagnosis.

    膠原病變是一種罕見的遺傳病,其特徵是膠原結構或代謝異常。通過全外顯子定序 (WES) 並輔以多重連接探針擴增技術(MLPA),我們確認6 例成骨不全 (OI) 的遺傳病因是為COL1A1的突變(p.T1298N、p.Q1280Pfs*51、 p.G557Vfs*23)和COL1A2的突變(c.1-1677_133-441del) 以及另外三例先天性脊椎骨骺發育不良有COL2A1的突變(p.G1041S、p.G654S、p.G441A)。在具有輕度 OI 表型但嚴重骨質疏鬆症的患者則發現同時存在COL1A1和WNT1突變。這些發現擴展了 I 型和 II 型膠原病變關於COL1A1、COL1A2、COL2A1的致病突變的光譜。儘管 WES 提供了一種快速準確的方法來識別遺傳原因,但在解釋結果時應牢記由於可變捕獲均勻性而導致檢測拷貝數多型性(CNV)的局限。我們證明了多種基因表徵技術可以提供準確有效的分子診斷。

  • 21. Enhanced output performance of ZnO thin film triboelectric nanogenerators by leveraging surface limited ga doping and insulting bulk.

    Lee, P.-C., Ou, Y.-C., Wang, R.-C., & Liu, C.-P. (2021). Nano Energy, 89, 106394. Cited this article

    Doping in semiconductors renders one serious drawback in triboelectric nanogenerator (TENG) as tribo-charges tend to leak for energy output. Therefore, a two-layer structure of Ga-doped ZnO/undoped-ZnO is proposed, where a ZnO thin film was first grown by sputter followed by Ga doping restricted to a shallow surface region by thermal diffusion. The results support that surface work function difference between two tribo-materials under the well preservation of trio-charges retention governs the charge transfer capability, where the output voltage and current of the ZnO film doped with 0.93 at% Ga can be enhanced by 16 and 13 times, respectively. Nevertheless, the larger work function of β-Ga2O3 than Ga-doped ZnO does not lead to larger output performance, implying the importance of carrier concentration in the performance of TENGs. We demonstrated a viable method to modify the work function of ZnO only confined to the surface through doping without disturbing the bulk, optimizing semiconductors for TENGs.


  • 22. Investigation of water bonding status of normal and psoriatic skin in vivo using diffuse reflectance spectroscopy.

    Yang, C.-C., Yen, Y.-Y., Hsu, C.-K., Cheng, N.-Y., Tzeng, S.-Y., Chou, S.-J., Chang, J.-M., & Tseng, S.-H. (2021). Scientific Reports, 11(1), 8901. Cited this article

    Psoriasis affects more than 125 million people worldwide, and the diagnosis and treatment efficacy evaluation of the disease mainly rely on clinical assessments that could be subjective. While the compromised epidermal barrier function has been taken as the major cause of clinical manifestation of skin inflammation of psoriasis, very few methods can be used to effectively evaluate this function. In this study, we investigate the near infrared spectroscopic features of psoriatic (n = 21) and normal (n = 21) skin that could link to the epidermal barrier function. From the DRS measurements, it was found that the water bonding status and light scattering properties of psoriasis are significantly different from those of uninvolved or normal skin. The connection between these parameters to the epidermal barrier function and morphology is discussed. Our results suggest that objective evaluation of epidermal barrier function of psoriasis could be achieved using a simple DRS system.


  • 23. Psychometric Evaluation of the Nurses Professional Values Scale-3: Indonesian Version.

    Asiandi, A., Erlina, M., Lin, Y.-H., & Huang, M.-C. (2021). International Journal of Environmental Research and Public Health, 18(16), 8810. Cited this article

    The Nurses Professional Values Scale-3 (NPVS-3) is an important instrument for measuring development and sustainability of professional values in nurses and nursing students. The purpose of this study was to evaluate the psychometric properties of NPVS-3, Indonesian version. The forward and backward translation was conducted to develop the NPVS3-I. Six hundred nurse participants filled this questionnaire. The 28-item scale of NPVS3-I was tested using EFA and CFA, applying the principal axis factoring extraction method and varimax with Kaiser normalization rotation method. Findings suggested sufficient content validity, construct validity, and reliability of the NPVS3-I. The I-CVI values ranged between 0.80 to 1.00 and the S-CVI was 0.99. Construct validity was supported with loading factors ranging from 0.49 to 0.84 for three factors (Caring, Activism, and Professionalism) and explain 65.40% variance. The NPVS3-I showed good internal consistency with Cronbach’s alpha= 0.97, and 0.94, 0.95, and 0.89 for Caring, Activism, and Professionalism, respectively.

    本研究旨在對轉譯之印尼版護理專業價值量表(NPVS3-I)進行信、效度檢測。問卷共28題,以分層隨機抽樣方式對3家醫院之600位護理師進行問卷調查。以探索性因素分析方式進行分析,結果顯示此問卷有良好的信效度,其中內容效度為0.8~1.0,建構效度確認3個因素(關懷、行動與專業)之負荷量為0.49~0.84,可解釋65.40%之變異量。問卷內在一致性Cronbach's α為0.97。

  • 24. Efficient DNA-Driven Nanocavities for Approaching Quasi-Deterministic Strong Coupling to a Few Fluorophores.

    Chan, W.-P., Chen, J.-H., Chou, W.-L., Chen, W.-Y., Liu, H.-Y., Hu, H.-C., Jeng, C.-C., Li, J.-R., Chen, C., & Chen, S.-Y. (2021). ACS Nano. Cited this article

    Strong coupling between an atom and a cavity has been utilized for quantum photonic devices such as deterministic single photon sources and quantum gates. The alignment between an atom and a cavity needs atom trapping and high vacuum equipment, which are hardly to be integrated into a microchip. Here, DNA strands connect a gold nanoparticle and a gold film to construct a nanocavity and simultaneously integrate a few fluorophores into the cavity to form a strong coupling unit. High cavity yield and strong coupling yield are achieved by this method. Combination of this method with e-beam lithography can further position strong coupling units on a specific location of a microchip. Furthermore, when we detune fluorophores embedded nanocavities, the polariton energy can fit into the curves derived from three sets of two-level systems, which indicates vibronic modes may be involved in the strong coupling systems.


  • 25. Systematic Identification of Protein Targets of Sub5 Using Saccharomyces cerevisiae Proteome Microarrays.

    Shah, P., & Chen, C.-S. (2021). International Journal of Molecular Sciences, 22(2), 760. Cited this article

    Antimicrobial peptides (AMPs) are intensively studied in terms of alternative drugs. Sub5 is a synthetic 12-mer AMP with substitutions of five amino acids of bactenecin 2A (Bac2A), a linear-ized bactenecin variant of bovine. Sub5 is highly effective against fungi with an ability to trans-locate cell membrane, but its targets are unknown. Systematic analysis of Sub5 targets will facilitate our understanding on its mechanism of action. In this study, we used high-throughput Saccharomyces cerevisiae proteome microarrays to explore the potential protein targets of Sub5. The screening results showed 128 potential protein targets of Sub5. Bioinformatics analysis of protein targets of Sub5 revealed significant gene ontology (GO) enrichment in actin related pro-cess of "actin filament-based process", "actin filament organization", "actin cortical patch or-ganization", regulation of "actin filament bundle assembly". Moreover, the other enriched cat-egories in GO enrichment mostly contained actin associate proteins. In total, 11 actin-associated proteins were identified in the protein targets of Sub5. Protein family (PFAM) enrichment anal-ysis shows protein domain enriched in actin binding, i.e., "Cytoskeletal-regulatory complex EF hand (helix E-loop-helix F motif)". Being consistent with GO analysis, Search Tool for the Re-trieval of Interacting Genes/Proteins (STRING) analysis of the protein targets of Sub5 showed ac-tin network with involvement of 15 protein targets. Along with actin-network, STRING analysis showed protein-protein interaction network in ribonucleoprotein, transcription and translation, chromosome, histone, and ubiquitin related, DNA repair, and chaperone. Multiple Expression motifs for Motif Elicitation (MEME) suite provided a consensus binding motif of [ED][ED]EEE[ED][ED][ED][ED][ED], in total of 75 protein targets of Sub5. This motif was present in 9 out of 15 actin-related proteins identified among protein targets of Sub5.

    抗菌肽已有許多研究。Sub5 是合成的抗菌肽,與Bac2A 差異五個胺基酸,有強效的抗黴能力,但其攻擊目標未知。系統性分析其攻擊目標將能增進我們對Sub5 作用機制的了解。在此,我們用酵母菌蛋白體微陣列晶片來分析其攻擊目標蛋白。結果顯示有128個目標蛋白。生物資訊分析發現這些蛋白顯著巨集在actin 相關的功能作用,功能區塊分析也與actin 結合功能有關。蛋白相互作用網路分析也有15個蛋白質和actin有關,其他也發現和轉譯、轉錄、染色體、DNA 等相關。序列分析也發現了在128個目標蛋白中有75個蛋白質有共同的胺基酸序列[ED][ED]EEE[ED][ED][ED][ED][ED], 此序列也存在於15個和actin 相關的目標蛋白質中的九個蛋白。

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